Microbiology Course Outline, Spring 2006

LINKS
MacKay's Home Page
Day/Date Lecture and discussion topics Reading assignments
in Bauman text
Cyber-Ed (available on
computers in Academic Commons--all optional)
Lab activities  (Lab manual page numbers are in parentheses)
Mon. & Tues.
2/13 & 2/14
A Brief History of Microbiology Chapter 1
(Chapter 4 helpful for lab)
Lecture will take up most of time today
Scope & group assignments
Wed. & Thurs.
2/15 & 2/16
The Chemistry of Microbiology Chapter 2 Biochemistry Use of the microscope (7-12)
Mon. & Tues.
2/20 & 2/21
NO VVC ON MONDAY, 2/20 0NLY (It's the President's Day holiday).  Tuesday section WILL meet on Tuesday.
Cell Structure and Function
Chapter 3
(Chapter 23 helpful for lab)
Cell structure and function
Inside the cell
The plasma membrane
(Protista helpful for lab)
Parasitology - Phylum Protozoa:  The Amebae (14-16)
Wed. & Thurs.
2/22 & 2/23
Cell Structure and Function, cont.
Characterizing and Classifying
Prokaryotes
Chapter 3
Chapter 11
Parasitology - Phylum Protozoa:  The Amebae (14-16) and The Flagellates (17-23)
Mon. & Tues.
2/27 & 2/28
LECTURE EXAM #1 (covers chapters 1, 2, and 3) Parasitology - Phylum Protozoa:  The Flagellates (17-23)
Wed. & Thurs.
3/1 & 3/2
Characterizing and Classifying Eukaryotes (specifically the Fungi), Pathogenic Fungi Chapter 12 (pp. 355-365),
Chapter 22
The Fungi Parasitology - Phylum Protozoa:  The Ciliates and the Apicomplexans (24-26, 27-31).  Inoculation of Sabaraud's dextrose agar for study of Fungi (Bring in moldy food from home) (56)
Mon. & Tues.
3/6 & 3/7
Microbial Metabolism Chapter 5 Cell respiration Tape mount and stain of hyphae and spore
structures with lactophenol cotton blue of group unknowns of fungi.  Inoculation of cornmeal with Candida albicans and C. glabrata (57-58).
Wed. & Thurs.
3/8 & 3/9
Microbial Metabolism, continued Chapter 5 Photosynthesis Continue identification of unknown fungi and observations of C. albicans and C. glabrata (59)
Parasitology - Phylum Platyhelminthes
Class Cestoda (34-39)
Mon. & Tues.
3/13 & 3/14
LECTURE EXAM 2.  (Covers chapters 11, 12, and 22)
Microbial Nutrition and Growth
Chapter 6 Phylum Platyhelminthes, Class Trematoda (40-45)
Wed. & Thurs.
3/15 & 3/16
Microbial Genetics Chapter 7 From DNA to protein Phylum Nematoda (46-52)
Mon. & Tues.
3/20 & 3/21
Recombinant DNA technology Chapter 8 Go over lab safety rules (3-4), aseptic technique (5), and lab growth media (6)
Determination of titer (60-63)
Wed. & Thurs.
3/22 & 3/23
LECTURE EXAM #3. (covers chapter 5)
Controlling Microbial Growth in the Body:  Antimicrobial Drugs
Chapter 10 Determination of titer, continued (60-63).  Be sure to work on titer practice problem sets (64-69) over the next week.
Mon. & Tues.
3/27 & 3/28
Infection, Infectious Diseases, and Epidemiology
Nonspecific Lines of Defense
Chapter 14
Chapter 15
Blood and immunity LAB EXAM #1 - Parasitology labs (through page 54)
Inoculate streak plates (70-71).  Take home plates for omnipresence of microorganisms (60)
Wed. & Thurs.
3/29 & 3/30
Specific Defense:  The Immune Response Chapter 16 Blood and immunity Examine streak plates and plates for omnipresence of microorganisms (60).  Smear preparation (72).  Simple stain (73-74). 
Mon. & Tues.
4/3 & 4/4
Specific Defense:  The Immune Response, continued Chapter 16 Blood and immunity LAB EXAM #2 - Aseptic technique, lab safety rules and determination of titer 
Wed. & Thurs.
4/5 & 4/6
LECTURE EXAM #4 (covers chapters 6, 7 and 8) Gram's stain (66-67)
Mon., Tues.,
Wed., Thurs.
4/10 - 4/13
NO VVC.  SPRING BREAK. NO VVC.  SPRING BREAK. NO VVC.  SPRING BREAK. NO VVC.  SPRING BREAK.
Mon. & Tues.
4/17 & 4/18
LECTURE EXAM #5 (covers chapters 10, 14, 15, and 16) Gram's stain, cont. (66-67). Capsule stain (70-71).  Endospore stain (72-73)
Wed. & Thurs.
4/19 & 4/20
Immunization and Immune Testing Chapter 17 Motility study (87).  Inoculate SIM media (88), Negative stain (77-78)
Mon. & Tues.
4/24 & 4/25
Hypersensitivities, Autoimmune Diseases, and Immune Deficiencies Chapter 18 Acid fast stain (74-75).  Metachromatic
granule stain (76-77).  Inoculation of selective and differential media (79)
Wed. & Thurs.
4/26 & 4/27
Hypersensitivities, Autoimmune Diseases, and Immune Deficiencies, continued Chapter 18 Utilization of unusual sources of N & C (80).  Hydrolysis of gelatin (83).  Hydrolysis of starch and casein (82)
Mon. & Tues.
5/1 & 5/2
LECTURE EXAM #6 (covers chapters 17 and 18) Fermentation of carbohydrates (91).  Production of hemolysin (113).  Detection of catalase (97).
Wed. & Thurs.
5/3 & 5/4
Pathogenic Gram-Positive Cocci Chapter 19 Viruses and bacteria Production of indole (99).  MRVP tests (102-103).  Production of H2S (98).  Reduction of nitrates (100-101).  Litmus milk reaction (93-94)
Mon. & Tues.
5/8 & 5/9
Pathogenic Gram-Positive Bacilli Chapter 19   LAB EXAM #3 - Staining technique and purpose, fungi labs (10/6-11/1)
Wed. & Thurs.
5/10 & 5/11
Pathogenic Gram-Negative Cocci Chapter 20   Bacterial analysis of water (110).  Evaluation of antibiotics (111-112)
Mon. & Tues.
5/15 & 5/16
Pathogenic Gram-Negative Bacilli Chapter 20   Bacterial analysis of water and evaluation of antibiotics, continued
Wed. & Thurs.
5/17 & 5/18
Mycoplasmas, Rickettsias,
Chlamydias, Spirochetes, and
Vibrios
Chapter 21 LAB EXAM #4 - Bacterial physiology (11/8-11/24)
Receive unknown bacterium; perform Gram's stain and make two new agar slants with it
Mon. & Tues.
5/22 & 5/23
LECTURE EXAM #7 (covers chapters 19, 20, and 21)   With unknown:  Inoculate carbohydrate fermentation, MR-VP, SIM, H2S, gelatin, indole, nitrate reduction, and litmus milk.  Gram's stain, negative stain, spore stain.
Wed. & Thurs.
5/24 & 5/25
Characterizing and Classifying Viruses, Viroids, and Prions Chapter 13 Viruses and bacteria With unknown:  Inoculate citrate, uric acid, catalase, production of hemolysin, hydrolysis of urea (96).  Capsule stain, motility study.
Mon. & Tues.
5/29 & 5/30
NO VVC ON MON., 5/29 ONLY (It's Memorial Day).  Tuesday section WILL meet on Tuesday, 5/30.
Pathogenic DNA viruses
Chapter 24 With unknown:  Inoculate detection of carboxylase enzymes (lysin and ornithine, 105), phenylalanine deaminase test (106).  Repeat tests and stains as needed, as long as media are available.
Wed. & Thurs.
5/31 & 6/1
Pathogenic DNA and RNA viruses Chapter 24 and 25 Work on unknown.  Make sure you have finished all stains and tests.
Mon. & Tues.
6/5 & 6/6
Pathogenic RNA Viruses Chapter 25 Finish all lab work with unknown; discard all media as per lab protocols.
Wed. & Thurs.
6/7 & 6/8
EXAM #8. (covers chapters 13, 24, 25) UNKNOWN DUE AT BEGINNING OF CLASS BEFORE THE EXAM TODAY
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