| Day/Date |
Lecture
and discussion topics |
Reading
assignments
in Bauman text |
Cyber-Ed
(available on
computers in Academic Commons--all optional) |
Lab
activities (Lab
manual page numbers are in parentheses) |
Mon.
& Tues.
2/13 & 2/14 |
A
Brief History of Microbiology |
Chapter
1
(Chapter 4 helpful for lab) |
|
Lecture
will take up most of time today
Scope & group assignments |
Wed.
& Thurs.
2/15 & 2/16 |
The
Chemistry of Microbiology |
Chapter
2 |
Biochemistry |
Use
of the microscope (7-12) |
Mon.
& Tues.
2/20 &
2/21 |
NO
VVC ON MONDAY, 2/20 0NLY (It's the President's Day holiday).
Tuesday section WILL meet on Tuesday.
Cell Structure and Function |
Chapter
3
(Chapter 23 helpful for lab) |
Cell
structure and function
Inside the cell
The plasma membrane
(Protista helpful for lab) |
Parasitology
- Phylum Protozoa: The
Amebae (14-16) |
Wed.
& Thurs.
2/22 & 2/23 |
Cell
Structure and Function, cont.
Characterizing and Classifying
Prokaryotes |
Chapter
3
Chapter 11 |
|
Parasitology
- Phylum Protozoa: The
Amebae (14-16) and The Flagellates (17-23) |
Mon.
& Tues.
2/27 & 2/28 |
LECTURE
EXAM #1 (covers chapters 1, 2, and 3) |
|
|
Parasitology
- Phylum Protozoa: The
Flagellates (17-23) |
Wed.
& Thurs.
3/1 & 3/2 |
Characterizing
and Classifying Eukaryotes (specifically the Fungi), Pathogenic
Fungi |
Chapter
12 (pp. 355-365),
Chapter 22 |
The
Fungi |
Parasitology
- Phylum Protozoa: The
Ciliates and the Apicomplexans (24-26, 27-31).
Inoculation of Sabaraud's dextrose agar for study of Fungi
(Bring in moldy food from home) (56) |
Mon.
& Tues.
3/6 & 3/7 |
Microbial
Metabolism |
Chapter
5 |
Cell
respiration |
Tape
mount and stain of hyphae and spore
structures with lactophenol cotton blue of group unknowns of
fungi. Inoculation of
cornmeal with Candida albicans
and C. glabrata (57-58). |
Wed.
& Thurs.
3/8 & 3/9 |
Microbial
Metabolism, continued |
Chapter
5 |
Photosynthesis |
Continue
identification of unknown fungi and observations of C.
albicans and C.
glabrata (59)
Parasitology - Phylum Platyhelminthes
Class Cestoda (34-39) |
Mon.
& Tues.
3/13 & 3/14 |
LECTURE
EXAM 2. (Covers
chapters 11, 12, and 22)
Microbial Nutrition and Growth |
Chapter
6 |
|
Phylum
Platyhelminthes, Class Trematoda (40-45) |
Wed.
& Thurs.
3/15 & 3/16 |
Microbial
Genetics |
Chapter
7 |
From
DNA to protein |
Phylum
Nematoda (46-52) |
Mon.
& Tues.
3/20 & 3/21 |
Recombinant
DNA technology |
Chapter
8 |
|
Go
over lab safety rules (3-4), aseptic technique (5), and lab growth
media (6)
Determination of titer (60-63) |
Wed.
& Thurs.
3/22 & 3/23 |
LECTURE
EXAM #3. (covers chapter 5)
Controlling Microbial Growth in the Body:
Antimicrobial Drugs |
Chapter
10 |
|
Determination
of titer, continued (60-63).
Be sure to work on titer practice problem sets (64-69) over
the next week. |
Mon.
& Tues.
3/27 & 3/28 |
Infection, Infectious Diseases, and Epidemiology
Nonspecific Lines of Defense |
Chapter
14
Chapter 15 |
Blood
and immunity |
LAB
EXAM #1 - Parasitology labs (through page 54)
Inoculate streak plates (70-71).
Take home plates for omnipresence of microorganisms (60) |
Wed.
& Thurs.
3/29 & 3/30 |
Specific
Defense: The Immune
Response |
Chapter
16 |
Blood
and immunity |
Examine
streak plates and plates for omnipresence of microorganisms (60).
Smear preparation (72).
Simple stain (73-74). |
Mon.
& Tues.
4/3 & 4/4 |
Specific
Defense: The Immune
Response, continued |
Chapter
16 |
Blood
and immunity |
LAB
EXAM #2 - Aseptic technique, lab safety rules and determination of
titer |
Wed.
& Thurs.
4/5 & 4/6 |
LECTURE
EXAM #4 (covers chapters 6, 7 and 8) |
|
|
Gram's
stain (66-67) |
Mon.,
Tues.,
Wed., Thurs.
4/10 - 4/13 |
NO
VVC. SPRING BREAK. |
NO
VVC. SPRING BREAK. |
NO
VVC. SPRING BREAK. |
NO
VVC. SPRING BREAK. |
Mon.
& Tues.
4/17 & 4/18 |
LECTURE
EXAM #5 (covers chapters 10, 14, 15, and 16) |
|
|
Gram's
stain, cont. (66-67). Capsule stain (70-71).
Endospore stain (72-73) |
Wed.
& Thurs.
4/19 & 4/20 |
Immunization
and Immune Testing |
Chapter
17 |
|
Motility
study (87). Inoculate
SIM media (88), Negative stain (77-78) |
Mon.
& Tues.
4/24 & 4/25 |
Hypersensitivities,
Autoimmune Diseases, and Immune Deficiencies |
Chapter
18 |
|
Acid
fast stain (74-75). Metachromatic
granule stain (76-77). Inoculation
of selective and differential media (79) |
Wed.
& Thurs.
4/26 & 4/27 |
Hypersensitivities,
Autoimmune Diseases, and Immune Deficiencies, continued |
Chapter
18 |
|
Utilization
of unusual sources of N & C (80).
Hydrolysis of gelatin (83).
Hydrolysis of starch and casein (82) |
Mon.
& Tues.
5/1 & 5/2 |
LECTURE
EXAM #6 (covers chapters 17 and 18) |
|
|
Fermentation
of carbohydrates (91). Production
of hemolysin (113). Detection
of catalase (97). |
Wed.
& Thurs.
5/3 & 5/4 |
Pathogenic
Gram-Positive Cocci |
Chapter
19 |
Viruses
and bacteria |
Production
of indole (99). MRVP
tests (102-103). Production
of H2S (98). Reduction
of nitrates (100-101). Litmus
milk reaction (93-94) |
Mon.
& Tues.
5/8 & 5/9 |
Pathogenic
Gram-Positive Bacilli |
Chapter
19 |
|
LAB
EXAM #3 - Staining technique and purpose, fungi labs (10/6-11/1) |
Wed.
& Thurs.
5/10 & 5/11 |
Pathogenic
Gram-Negative Cocci |
Chapter
20 |
|
Bacterial
analysis of water (110). Evaluation
of antibiotics (111-112) |
Mon.
& Tues.
5/15 & 5/16 |
Pathogenic
Gram-Negative Bacilli |
Chapter
20 |
|
Bacterial
analysis of water and evaluation of antibiotics, continued |
Wed.
& Thurs.
5/17 & 5/18 |
Mycoplasmas,
Rickettsias,
Chlamydias, Spirochetes, and
Vibrios |
Chapter
21 |
|
LAB
EXAM #4 - Bacterial physiology (11/8-11/24)
Receive unknown bacterium; perform
Gram's stain and make two new agar slants with it |
Mon.
& Tues.
5/22 & 5/23 |
LECTURE
EXAM #7 (covers chapters 19, 20, and 21) |
|
|
With
unknown: Inoculate
carbohydrate fermentation, MR-VP, SIM, H2S, gelatin, indole,
nitrate reduction, and litmus milk.
Gram's stain, negative stain, spore stain. |
Wed.
& Thurs.
5/24 & 5/25 |
Characterizing
and Classifying Viruses, Viroids, and Prions |
Chapter
13 |
Viruses
and bacteria |
With
unknown: Inoculate
citrate, uric acid, catalase, production of hemolysin, hydrolysis
of urea (96). Capsule stain, motility study. |
Mon.
& Tues.
5/29 &
5/30 |
NO
VVC ON MON., 5/29 ONLY (It's Memorial Day).
Tuesday section WILL meet on Tuesday, 5/30.
Pathogenic DNA viruses |
Chapter
24 |
|
With
unknown: Inoculate
detection of carboxylase enzymes (lysin and ornithine, 105),
phenylalanine deaminase test (106).
Repeat tests and stains as needed, as long as media are
available. |
Wed.
& Thurs.
5/31 & 6/1 |
Pathogenic
DNA and RNA viruses |
Chapter
24 and 25 |
|
Work
on unknown. Make sure
you have finished all stains and tests. |
Mon.
& Tues.
6/5 & 6/6 |
Pathogenic
RNA Viruses |
Chapter
25 |
|
Finish
all lab work with unknown; discard all media as per lab protocols. |
Wed.
& Thurs.
6/7 & 6/8 |
EXAM
#8. (covers chapters 13, 24, 25) |
|
|
UNKNOWN
DUE AT BEGINNING OF CLASS BEFORE THE EXAM TODAY |